A fully synthetic human Fab antibody library based on fixed VH/VL framework pairings with favorable biophysical properties
نویسندگان
چکیده
منابع مشابه
Construction of a human recombinant polyclonal Fab fragment antibody library using peripheral blood lymphocytes of snake bitten victims
Human snake bitten poisoning is a serious threat in many tropical and subtropical countries such as Iran. The best acceptable treatment of envenomated humans is antivenoms; however they have a series of economic and medical problems and need more improvements. In this study a combinatorial human immunoglobulin gene library against some of Iranian snakes venoms was constructed. Total RNA prepare...
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There are great demands on the stability, expression yield and resistance to aggregation of antibody fragments. To untangle intrinsic domain effects from domain interactions, we present first a systematic evaluation of the isolated human immunoglobulin variable heavy (V(H)) and light (V(L)) germline family consensus domains and then a systematic series of V(H)-V(L) combinations in the scFv form...
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Osteosarcoma is the commonest type of primary malignant bone tumor, frequently found in adolescents at sites of rapid bone growth. Despite current management protocols, up to half of the patients succumb to this disease. Moreover, there is no well-characterized molecular marker for diagnosis and prognosis. Since phage display methodology allows the selection of human antibody fragments with pot...
متن کاملconstruction of a human recombinant polyclonal fab fragment antibody library using peripheral blood lymphocytes of snake bitten victims
human snake bitten poisoning is a serious threat in many tropical and subtropical countries such as iran. the best acceptable treatment of envenomated humans is antivenoms; however they have a series of economic and medical problems and need more improvements. in this study a combinatorial human immunoglobulin gene library against some of iranian snakes venoms was constructed. total rna prepare...
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By analyzing the human antibody repertoire in terms of structure, amino acid sequence diversity and germline usage, we found that seven V(H) and seven V(L) (four Vkappa and three Vlambda) germline families cover more than 95 % of the human antibody diversity used. A consensus sequence was derived for each family and optimized for expression in Escherichia coli. In order to make all six compleme...
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ژورنال
عنوان ژورنال: mAbs
سال: 2013
ISSN: 1942-0862,1942-0870
DOI: 10.4161/mabs.24218